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PEGylated adenovirus vectors containing RGD peptides on the tip of PEG show high transduction efficiency and antibody evasion ability by Yusuke Eto; Jian‐Qing Gao; Fumiko Sekiguchi; Shinnosuke Kurachi; Kazufumi Katayama; Mitsuko Maeda; Koichi Kawasaki; Hiroyuki Mizuguchi; Takao Hayakawa; Yasuo Tsutsumi; Tadanori Mayumi; Shinsaku Nakagawa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is PEGylated adenovirus vectors containing RGD peptides on the tip of PEG show high transduction efficiency and antibody evasion ability about?

## Abstract ## Background PEGylation of adenovirus vectors (Ads) is an attractive strategy in gene therapy. Although many types of PEGylated Ad (PEG‐Ads), which exhibit antibody evasion activity and long plasma half‐life, have been developed, their entry into cells has been prevented by steric hindrance by polyethylene glycol (PEG) chains. Likewise, sufficient gene expression for medical treatment could not be achieved. ## Methods A set of PEG‐Ads, which have different PEG modification rates, was constructed, and gene expression was evaluated using A549 cells. A novel PEGylated Ad (RGD‐PEG‐Ad), which contained RGD (Arg‐Gly‐Asp) peptides on the tip of PEG, was developed. We evaluated gene expression both in Coxsackie‐adenovirus receptor (CAR)‐positive as well as ‐negative cells, and __in vivo__ gene expression was also determined. Furthermore, the antibody evasion ability and the specificity of infection exhibited by this RGD‐PEG‐Ad were also evaluated. ## Results Whereas PEG‐Ads decreased gene expression in CAR‐positive cells, RGD‐PEG‐Ad enhanced gene expression notably, to a level about 200‐fold higher than that of PEG‐Ads. Moreover, gene expression of RGD‐PEG‐Ad was almost equal

Who reads PEGylated adenovirus vectors containing RGD peptides on the tip of PEG show high transduction efficiency and antibody evasion ability?

Author

Yusuke Eto; Jian‐Qing Gao; Fumiko Sekiguchi; Shinnosuke Kurachi; Kazufumi Katayama; Mitsuko Maeda; Koichi Kawasaki; Hiroyuki Mizuguchi; Takao Hayakawa; Yasuo Tsutsumi; Tadanori Mayumi; Shinsaku Nakagawa

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)