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Mapping of Neuroinflammation-induced Hypoxia in the Spinal Cord Using Optoacoustic Imaging by Ramos-Vega, Marta (author);Kjellman, Pontus (author);Todorov, Mihail Ivilinov (author);Kylkilahti, Tekla Maria (author);Bäckström, B. Thomas (author);Ertürk, Ali (author);Madsen, Chris Denis (author);Lundgaard, Iben (author) is a Medicine article available to read on EtoBox.

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## Abstract Recent studies suggest that metabolic changes and oxygen deficiency in the central nervous system play an important role in the pathophysiology of multiple sclerosis (MS). In our present study, we investigated the changes in oxygenation and analyzed the vascular perfusion of the spinal cord in a rodent model of MS. We performed multispectral optoacoustic tomography of the lumbar spinal cord before and after an oxygen enhancement challenge in mice with experimental autoimmune encephalomyelitis (EAE), a model for MS. In addition, mice were transcardially perfused with lectin to label the vasculature and their spinal columns were optically cleared, followed by light sheet fluorescence microscopy. To analyze the angioarchitecture of the intact spine, we used VesSAP, a novel deep learning-based framework. In EAE mice, the spinal cord had lower oxygen saturation and hemoglobin concentration compared to healthy mice, indicating compromised perfusion of the spinal cord. Oxygen administration reversed hypoxia in the spinal cord of EAE mice, although the ventral region remained hypoxic. Additionally, despite the increased vascular density, we report a reduction in length and comp

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Author

Ramos-Vega, Marta (author);Kjellman, Pontus (author);Todorov, Mihail Ivilinov (author);Kylkilahti, Tekla Maria (author);Bäckström, B. Thomas (author);Ertürk, Ali (author);Madsen, Chris Denis (author);Lundgaard, Iben (author)

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Medicine (Health Sciences)