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Isolation and Endotoxin Activities of Lipopolysaccharides from Cyanobacterial Cultures and Complex Water Blooms and Comparison with the Effects of Heterotrophic Bacteria and Green Alga by Kateřina Bernardová; Pavel Babica; Blahoslav Maršálek; Luděk Bláha is a Environmental Science article available to read on EtoBox.

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## Abstract Massive cyanobacterial water blooms are serious environmental and health problems worldwide. While some cyanobacterial toxins such as peptide microcystins have been investigated extensively, other toxic components of cyanobacteria (e.g. lipopolysaccharides, LPS) are poorly understood. The present study characterized endotoxin activities of LPS isolated from (i) laboratory cyanobacterial cultures, (ii) cyanobacterial water bloom samples dominated by __Microcystis__ sp., __Planktothrix__ sp., __Aphanizomenon__ sp. and __Anabaena__ sp., (iii) heterotrophic Gram‐negative bacteria __Escherichia coli, Kluyvera intermedia, Pseudomonas putida__ and __Pseudomonas fluorescens__ and (iv) green alga __Pseudokirchneriella subcapitata__. Toxicity results derived with __Limulus__ amebocyte lysate assay (LAL‐test) showed that endotoxin activities of LPS from both cyanobacteria and heterotrophic bacteria were comparable and the values were within a similar range (1 × 10^3^–1 × 10^6^ Endotoxin Units, EU, per mg of isolated LPS). The highest activities among the cyanobacterial samples were observed in the __Aphanizomenon__ sp. dominated water bloom. The results also suggest generally high

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Author

Kateřina Bernardová; Pavel Babica; Blahoslav Maršálek; Luděk Bláha

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0260-437X)

Published

2007

Language

EN

Field

Environmental Science (Physical Sciences)