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Rapid identification of antigenic T-cell epitopes by extracellular acidification rate signals by Subhashini Arimilli; Maureen C. Howard; Carol C. Nacy; Shrikant V. Deshpande is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Rapid identification of antigenic T-cell epitopes by extracellular acidification rate signals about?

We used a silicon-based biosensor, a microphysiometer, to measure real-time extracellular acidification rate signals associated with T lymphocyte responses to peptide ligands interacting with the T-cell receptor (TCR). We compared these effector responses with those of interferon-␥ (IFN-␥) production, and T-cell proliferation. Within minutes, major histocompatibility complex (MHC)-bound peptides on antigen-presenting cells (APCs) engaged the TCR to increase acidification rates of the extracellular media was measured by microphysiometer. We exposed two myelin peptide-specific human T-cell clones, MSF132E11 (DRB1\*1501 restricted) and TOM3A6 (DRB5\*0101 restricted), to truncated analogues of the parent MBP 84-102 peptide, in the presence of MHC restricted human antigen-presenting cells, and measured the extracellular acidification rate signal changes, IFN-␥ production and T-cell proliferation. The core epitopes recognized by these clones were identified by microphysiometer and found to be MBP 88 -100 and MBP 91-100, respectively. These epitopes were identical to those identified by the IFN-␥ and proliferation assays. We conclude that measurement of real-time extracellular acidificati

Who reads Rapid identification of antigenic T-cell epitopes by extracellular acidification rate signals?

Author

Subhashini Arimilli; Maureen C. Howard; Carol C. Nacy; Shrikant V. Deshpande

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)