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Glucosamine:fructose-6-phosphate aminotransferase: gene characterization, chitin biosynthesis and peritrophic matrix formation in Aedes aegypti by N. Kato; R. Dasgupta; C. T. Smartt; B. M. Christensen is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Glucosamine:fructose-6-phosphate aminotransferase: gene characterization, chitin biosynthesis and peritrophic matrix formation in Aedes aegypti about?

## Abstract Glucosamine:fructose‐6‐phosphate aminotransferase (GFAT) catalyses the formation of glucosamine 6‐phosphate and is the first and rate‐limiting enzyme of the hexosamine biosynthetic pathway. The final product of the hexosamine pathway, UDP‐__N__‐acetyl glucosamine, is an active precursor of numerous macromolecules containing amino sugars, including chitin in fungi and arthropods. Chitin is one of the essential components of insect cuticle and peritrophic matrix. The peritrophic matrix is produced in the midgut of mosquitoes in response to bloodfeeding, and may affect vector competence by serving as a physical barrier to pathogens. It is hypothesized that GFAT plays a regulatory role in biosynthesis of chitin and peritrophic matrix formation in insects. We cloned and sequenced the GFAT gene (__AeGfat‐1__) and its 5′ regulatory region from __Aedes aegypti__. There is no intron in __AeGfat‐1__ and there are two potential transcription start sites. __AeGfat‐1__ cDNA is 3.4 kb in length and its putative translation product is 75.4 kDa. The amino acid sequence of GFAT is highly conserved in lower and higher eukaryotes, as well as in bacteria. __AeGfat‐1__ message is constituti

Who reads Glucosamine:fructose-6-phosphate aminotransferase: gene characterization, chitin biosynthesis and peritrophic matrix formation in Aedes aegypti?

Author

N. Kato; R. Dasgupta; C. T. Smartt; B. M. Christensen

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0962-1075)

Published

2002

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)