About this Biochemistry, Genetics and Molecular Biology article
G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis by Jiaping Chen; Rong Zhao; Yangwei Wang; Han Xiao; Wei Lin; Mingxin Diao; Shiwen He; Peiyuan Mei; Yongde Liao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
## Background Lung cancer is the leading cause of cancer-related death worldwide. The sex differences in the occurrence and fatality rates of non-small cell lung cancer (NSCLC), along with its association with estrogen dependence, suggest that estrogen receptors (ERs) contribute to the development of NSCLC. However, the influence of G protein-coupled estrogen receptor (GPER1) on NSCLC remains to be determined. Escape from ferroptosis is one of the hallmarks of tumor discovered in recent years. In this context, the present study evaluated whether GPER1 promotes NSCLC progression by preventing ferroptosis, and the underlying mechanism through which GPER1 protects against ferroptosis was also explored. ## Methods The effects of GPER1 on the cytotoxicity of H~2~O~2~, the ferroptosis inducer RSL3, and Erastin were assessed using the CCK8 assay and plate cloning. Lipid peroxidation levels were measured based on the levels of MDA and BODIPYTM581/591C11. GPER1 overexpression and knockdown were performed and G1 was used, and the expression of SCD1 and PI3K/AKT/mTOR signaling factors was measured. Immunofluorescence analysis and immunohistochemistry were performed on paired specimens to meas
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- Author
- Jiaping Chen; Rong Zhao; Yangwei Wang; Han Xiao; Wei Lin; Mingxin Diao; Shiwen He; Peiyuan Mei; Yongde Liao
- Publisher
- BioMed Central
- Published
- 2024
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)