Can I read [33] Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension on EtoBox?

[33] Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension by Seth Stern; Danesh Moazed; Harry F. Noller is a book available to read on EtoBox.

What is [33] Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension about?

PROBING MONITORED BY PRIMER EXTENSION 48 1 Applications This method has been used to probe higher-order (i.e., secondary and tertiary) structure in 16S ribosomal RNA using DEP, 2 and to study thermal denaturation of specific regions of 16S rRNA.~2 Another application is in the preparation of defined fragments of large RNA molecules for biochemical or physical studies. Draper and co-workers ~a have developed a related method, in which a specific restriction fragment cloned into an M 13 vector is used as a source of the complementary DNA strand. With the availability of large quantities of synthetic DNA oligomers, preparation of specific RNA fragments in milligram amounts for physical studies should also be possible.

Author

Seth Stern; Danesh Moazed; Harry F. Noller

Published

1988

Language

EN