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A combined free-flow electrophoresis and DIGE approach to identify proteins regulated by butyrate in HT29 cells by Kim Y. C. Fung; Chris Cursaro; Tanya Lewanowitsch; Gemma V. Brierley; Shaun R. McColl; Trevor Lockett; Richard Head; Peter Hoffmann; Leah Cosgrove is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Many biologically active agents exert a pleiotropic response in cells and tissues. This presents challenges in descriptive and comparative analysis of the proteome in response to these agents. Although free‐flow electrophoresis has been applied in a number of proteomic studies as a protein separation technique, the combination of free‐flow electrophoresis and DIGE has not yet been investigated for comparative proteomic analysis. In this study, we have compared the effects of butyrate on HT29 colorectal cancer cells with a particular focus on apoptosis and describe the utility of a novel approach combining free‐flow electrophoresis with DIGE to identify differentially expressed proteins. We verify the results obtained by the combined free‐flow electrophoresis and DIGE approach with Western blot analysis of selected proteins. We also report for the first time the regulation of a number of proteins by butyrate in HT29 colorectal cells including peptidyl‐prolyl __cis‐trans__ isomerase A (cyclophilin A) and profilin‐1.
Who reads A combined free-flow electrophoresis and DIGE approach to identify proteins regulated by butyrate in HT29 cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kim Y. C. Fung; Chris Cursaro; Tanya Lewanowitsch; Gemma V. Brierley; Shaun R. McColl; Trevor Lockett; Richard Head; Peter Hoffmann; Leah Cosgrove
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)