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What is Tail PCR Protocol and Techniques about?

TAIL-PCR is a technique for obtaining DNA sequence from flanking regions of a known sequence. It involves 3 rounds of PCR (primary, secondary, tertiary) using specific primers spaced out from the known sequence along with degenerate arbitrary primers. Between rounds, products are diluted 100x and used as templates. The technique exploits the ability of Taq polymerase to extend from nonspecifically annealed primers at low annealing temperatures. After each round, products shift to higher sizes corresponding

Author

Felipe Zapata

Language

EN