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Quantitative Proteomics Reveals Common and Unique Molecular Mechanisms Underlying Beneficial Effects of Caffeine and Trigonelline on Human Hepatocytes by Paleerath Peerapen; Chanettee Chanthick; Visith Thongboonkerd is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Quantitative Proteomics Reveals Common and Unique Molecular Mechanisms Underlying Beneficial Effects of Caffeine and Trigonelline on Human Hepatocytes about?

Caffeine and trigonelline are the major bioactive compounds in coffee. Caffeine alone or combined with other coffee compounds shows hepatoprotective effects. However, molecular mechanisms underlying such hepatoprotective effects remain unclear. We therefore addressed molecular effects of caffeine and trigonelline on human hepatocytes using quantitative proteomics followed by bioinformatic analyses to obtain topological and functional significance. HepG2 cells were treated with 100 μM caffeine or trigonelline for 24-h and evaluated by quantitative proteomics using nanoLC-ESI-LTQ-Orbitrap MS/MS. A total of 26 and 25 significantly altered proteins were identified in caffeine-treated and trigonelline-treated cells, respectively, compared with control cells. Topological analyses revealed that ribosomal and translation regulatory proteins predominantly served as the hub proteins associated with protein clusters. Functional analyses also revealed that these two bioactive compounds shared some molecular mechanisms via induction of translational processes. There were also other unique molecular functions and biological processes triggered or suppressed by either caffeine or trigonelline. Th

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Author

Paleerath Peerapen; Chanettee Chanthick; Visith Thongboonkerd

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)