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β-Sitosterol Targets Glucocorticoid Receptor to Reduce Airway Inflammation and Remodeling in Allergic Asthma by Jianfeng Xu; Lei Yang; Tiantian Lin is a Medicine article available to read on EtoBox.

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Introduction In most asthma patients, symptoms are controlled by treatment with glucocorticoid, but long-term or high-dose use can produce adverse effects. Therefore, it is crucial to find new therapeutic strategies. β-sitosterol could suppress type II inflammation in ovalbumin (OVA)-induced mice, but its mechanisms have remained unclear. Methods A binding activity of β-sitosterol with glucocorticoid receptor (GR) was analyzed by molecular docking. Human bronchial epithelial cells (BEAS-2B) and human bronchial smooth muscle cells (HBSMC) were treated with different concentrations (0, 1, 5, 10, 20, and 50 μg/mL) of β-sitosterol for suitable concentration selection. In transforming growth factor (TGF)-β1 treated BEAS-2B and HBSMC, cells were treated with 20 μg/mL β-sitosterol or dexamethasone (Dex) to analyze its possible mechanism. In OVA-induced mice, 2.5 mg/kg β-sitosterol or Dex administration was performed to analyze the therapeutic mechanism of β-sitosterol. A GR antagonist RU486 was used to confirm the mechanism of β-sitosterol in the treatment of asthma. Results A good binding of β-sitosterol to GR (score = −8.2 kcal/mol) was found, and the GR expression was upregulated with

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Author

Jianfeng Xu; Lei Yang; Tiantian Lin

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)