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Impact of the coxsackie and adenovirus receptor (CAR) on glioma cell growth and invasion: Requirement for the C-terminal domain by Kuo-Cheng Huang; Meric Altinoz; Karolina Wosik; Nancy Larochelle; Zafiro Koty; Lixia Zhu; Paul C. Holland; Josephine Nalbantoglu is a Medicine article available to read on EtoBox.

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## Abstract Expression of the coxsackie and adenovirus receptor (CAR) is downregulated in malignant glioma cell lines and is barely detectable in high‐grade primary astrocytoma (glioblastoma multiforme). We determined the effect of forced CAR expression on the invasion and growth of the human glioma cell line U87‐MG, which does not express any CAR. Although retrovirally mediated expression of full‐length CAR in U87‐MG cells did not affect monolayer growth __in vitro__, it did reduce glioma cell invasion in a 3‐dimensional spheroid model. Furthermore, in xenograft experiments, intracerebral implantation of glioma cells expressing full‐length CAR resulted in tumors with a significantly reduced volume compared to tumors generated by control vector‐transduced U87‐MG cells. In contrast, U87‐MG cells expressing transmembrane CAR with a deletion of the entire cytoplasmic domain (except for the first 2 intracellular juxtamembrane cysteine amino acids) had rates of invasion and tumor growth that were similar to those of the control cells. This difference in behavior between the 2 forms of CAR was not due to improper cell surface localization of the cytoplasmically deleted CAR as determined

Who reads Impact of the coxsackie and adenovirus receptor (CAR) on glioma cell growth and invasion: Requirement for the C-terminal domain?

Author

Kuo-Cheng Huang; Meric Altinoz; Karolina Wosik; Nancy Larochelle; Zafiro Koty; Lixia Zhu; Paul C. Holland; Josephine Nalbantoglu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2004

Language

EN

Field

Medicine (Health Sciences)