Can I read Free fetal DNA in maternal circulation: a potential prognostic marker for chromosomal abnormalities? on EtoBox?

Free fetal DNA in maternal circulation: a potential prognostic marker for chromosomal abnormalities? by Ageliki Gerovassili; Chad Garner; Kypros H. Nicolaides; Swee Lay Thein; David C. Rees is a Medicine article available to read on EtoBox.

What is Free fetal DNA in maternal circulation: a potential prognostic marker for chromosomal abnormalities? about?

## Abstract ## Objectives Previous studies on the association of fetal cell‐free (cf)DNA levels in maternal circulation have produced conflicting results but the sample sizes were small and based on archived material. We aimed to quantify the levels of fetal and total cfDNA on prospectively collected samples, to understand their correlation with other variables and to clarify their diagnostic value. ## Methods DNA from pre‐CVS maternal plasma was extracted from 264 controls, 72 trisomy 21, 24 trisomy 18, 12 trisomy 13, 16 Turner's syndrome and 8 triploidy first‐trimester pregnancies and quantified using real‐time PCR. β‐globin was used to determine total cfDNA levels and __DYS14__ and __SRY__ assays to determine fetal cfDNA levels. ## Results Fetal cfDNA levels (__DYS14__) showed correlation with crown rump length (CRL) (__p__ = 0.004), BMI (__p__ = 0.01) and storage time (__p__ = 0.007) while there was an inverse correlation of total cfDNA levels with nuchal translucency (NT) (__p__ = 0.001). No significant difference was observed between the levels of fetal cfDNA in controls and aneuploidy cases. ## Conclusion Quantification of fetal and total cfDNA in maternal circulation showed

Who reads Free fetal DNA in maternal circulation: a potential prognostic marker for chromosomal abnormalities??

Author

Ageliki Gerovassili; Chad Garner; Kypros H. Nicolaides; Swee Lay Thein; David C. Rees

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0197-3851)

Published

2006

Language

EN

Field

Medicine (Health Sciences)