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A genetic screen to isolate genes regulated by the yeast CCAAT‐box binding protein Hap2p by Van‐Dinh Dang; Michèle Valens; Monique Bolotin‐Fukuhara; Bertrand Daignan‐Fornier is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A genetic screen to isolate genes regulated by the yeast CCAAT‐box binding protein Hap2p about?

We have developed a screening method to isolate yeast genes regulated by a specific transcription activator. The screen is based on the use of expression libraries in which the lacZ reporter gene is placed under control of yeast regulatory elements. Two partially representative libraries, constructed by different methods, were used to isolate genes regulated by the yeast CCAAT-box binding protein Hap2p. Among 26 fusions shown to be regulated by Hap2p only CYTl was known to be regulated by this activator. Sequence analysis revealed that most of the remaining regulated fusions are in new yeast genes, while some are in previously characterized yeast genes (PTPI, RPM2, SDHI). Optimal expression of these three genes also requires Hap3p and Hap4p and is regulated by carbon source. Hap2p was known to regulate expression of genes involved in Krebs cycle, electron transport and heme biosynthesis. Our results suggest that Hap2p could play a more general role by regulating other mitochondrial processes such as protein import and phosphate transport (PTPI) or maturation of mitochondria1 tRNAs (RPM2). Among the remaining regulated fusions, two of them correspond to open reading frames (ORFs) on

Who reads A genetic screen to isolate genes regulated by the yeast CCAAT‐box binding protein Hap2p?

Author

Van‐Dinh Dang; Michèle Valens; Monique Bolotin‐Fukuhara; Bertrand Daignan‐Fornier

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)