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Spatio‐temporal pattern of MAP kinase activation in embryos of the ascidian Halocynthia roretzi by Hiroki Nishida is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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To understand developmental mechanisms, it is important to know when and where signaling pathways are activated. The spatio‐temporal pattern of activation of mitogen‐activated protein kinase (MAPK/ERK) was investigated during embryogenesis of the ascidian __Halocynthia roretzi__, using an antibody specific to the activated form of MAPK. During cleavage stages, activated MAPK was transiently observed in nuclei of the precursor blastomeres of endoderm, notochord, mesenchyme, brain, secondary muscle, trunk lateral cells and trunk ventral cells. These sites of MAPK activation are consistent with results of previous studies that have analyzed the embryonic induction of various tissues, and with results of inhibition of MAPK kinase (MEK) in ascidians. Activation of MAPK in notochord and mesenchyme blastomeres was observed in a short period in a single cell cycle. In contrast, in brain and secondary muscle lineages, MAPK activation spanned two or three cell cycles, and upon each cleavage, MAPK was asymmetrically activated in only one of the two daughter cells that remained brain or secondary muscle lineages. During later stages, MAPK activation was predominantly observed in the central ne

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Author

Hiroki Nishida

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0012-1592)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)