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Can I read Induction of anti-tumor immunity by intrasplenic administration of a carcinoembryonic antigen DNA vaccine on EtoBox?
Induction of anti-tumor immunity by intrasplenic administration of a carcinoembryonic antigen DNA vaccine by Stephen A. White; Albert F. LoBuglio; Ramin B. Arani; Mary J. Pike; Susan E. Moore; Daunte L. Barlow; Robert M. Conry is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Induction of anti-tumor immunity by intrasplenic administration of a carcinoembryonic antigen DNA vaccine about?
Background We have previously reported that intramuscular, intradermal or epidermal gene gun administration of a plasmid encoding carcinoembryonic antigen (CEA) under transcriptional regulatory control of the cytomegalovirus (CMV) early promoter/enhancer elicits CEA-speci®c humoral and cellular immune responses in mice with resultant immunoprotection against challenge with syngeneic, CEA-expressing colon adenocarcinoma cells. ## Methods In the present work, we examine the ability of this DNA vaccine construct (pCEA) to elicit CEA-speci®c immunity following intrasplenic administration. Groups of mice were immunized with pCEA by intrasplenic or intramuscular injection. Six weeks later, mice were evaluated for the presence of anti-CEA humoral responses and were challenged with syngeneic, CEAexpressing colon carcinoma cells. Results Intrasplenic administration of pCEA produced a frequency of CEA-speci®c antibody responses comparable to that elicited by intramuscular pCEA inoculation. Both intrasplenic and intramuscular administration of pCEA generated IgG2a antibody responses to CEA, consistent with the induction of T helper-1-biased immune responses. In addition, partial immunoprotect
Who reads Induction of anti-tumor immunity by intrasplenic administration of a carcinoembryonic antigen DNA vaccine?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Stephen A. White; Albert F. LoBuglio; Ramin B. Arani; Mary J. Pike; Susan E. Moore; Daunte L. Barlow; Robert M. Conry
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)