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Can I read Identification of point mutations in a putative carboxylesterase and their association with acaricide resistance in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) on EtoBox?
Identification of point mutations in a putative carboxylesterase and their association with acaricide resistance in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) by Milla Alves Baffi; Guilherme Rocha Lino de Souza; Carlos Ueira Vieira; Cristina Soares de Sousa; Luiz Ricardo Gourlart; Ana Maria Bonetti is a Immunology and Microbiology article available to read on EtoBox.
What is Identification of point mutations in a putative carboxylesterase and their association with acaricide resistance in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) about?
Chemical control based on the use of pyrethroid and organophosphate compounds has selected resistant genotypes in populations of Rhipicephalus (Boophilus) microplus. Point mutations in esterase-encoding genes represent one of the main resistance mechanisms in this species. In this study, the PCR-RFLP ( polymerase chain reaction-restriction fragment length polymorphism) technique was used to investigate the presence of mutations in a fragment of a putative carboxylesterase in a population of ticks with a history of resistance. The digestion of a fragment of 372 pb with EcoRI revealed three genotypes: W, H and M, observed in different frequencies. The homozygous wild-type genotype (W) was detected only in sensitive strains, with high frequency. The heterozygous genotype (H) was observed in all the strains, albeit with higher frequency in the strains with a moderate resistance, while the homozygous mutant genotype (M) was found only in the moderate resistant strain and resistant strains, with higher frequency in the resistant strains. A comparison of the sequences indicated the presence of other mutations, besides EcoRI polymorphism in the moderate resistant and resistant strains. Als
Who reads Identification of point mutations in a putative carboxylesterase and their association with acaricide resistance in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Milla Alves Baffi; Guilherme Rocha Lino de Souza; Carlos Ueira Vieira; Cristina Soares de Sousa; Luiz Ricardo Gourlart; Ana Maria Bonetti
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)
- Published
- 2007
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)