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The Roles of Connective Tissue Growth Factor and Integrin-linked Kinase in High Glucose-induced Phenotypic Alterations of Podocytes by Hou-Yong Dai; Min Zheng; Lin-Li Lv; Ri-Ning Tang; Kun-Ling Ma; Dan Liu; Min Wu; Bi-Cheng Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The Roles of Connective Tissue Growth Factor and Integrin-linked Kinase in High Glucose-induced Phenotypic Alterations of Podocytes about?
## Abstract Emerging evidence has suggested that podocytes undergo epithelial–mesenchymal transition (EMT) in diabetic nephropathy (DN). Connective tissue growth factor (CTGF) and integrin‐linked kinase (ILK) are involved in the progression of DN. However, the underlying mechanisms of EMT are not well understood. The study aimed to investigate the roles of CTGF and ILK in high glucose‐induced phenotypic alterations of podocytes and determine whether ILK signaling is downstream of CTGF. The epithelial marker of nephrin and the mesenchymal marker of desmin were investigated by real‐time RT‐PCR and Western blotting. The results demonstrated that podocytes displayed a spreading, arborized morphology in normal glucose, whereas they had a cobblestone morphology in high glucose conditions, accompanied by decreased nephrin expression and increased desmin expression, suggesting podocytes underwent EMT. In response to high glucose, CTGF and ILK expression in podocytes were increased in a dose‐ and time‐dependent manner, whereas the increase did not occur in the osmotic control. Furthermore, the inhibition of CTGF with anti‐CTGF antibody prevented the phenotypic transition, as demonstrated by
Who reads The Roles of Connective Tissue Growth Factor and Integrin-linked Kinase in High Glucose-induced Phenotypic Alterations of Podocytes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hou-Yong Dai; Min Zheng; Lin-Li Lv; Ri-Ning Tang; Kun-Ling Ma; Dan Liu; Min Wu; Bi-Cheng Liu
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)