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Regionally specific distribution of the binding of anti-glutamine synthetase and anti-S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn by Silvana Allodi; Cristine M. Bressan; Sergio L. Carvalho; Leny A. Cavalcante is a Neuroscience article available to read on EtoBox.

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## Abstract We previously characterized some crustacean glial cells by markers such as 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase and glial fibrillary acidic protein. Here we use antibodies against glutamine synthetase full‐length molecule (anti‐GS/FL), a GS C‐terminal peptide (anti‐GS/20aa‐C), and brain S100 (anti‐S100), as well as the binding of the insect glia and rat astrocytic marker __Datura stramonium__ lectin (DSL), in the optic lobe of the prawn __Macrobrachium rosenbergii__. All markers label the lamina ganglionaris cartridge region (lighter: anti‐GS/FL; heavier: DSL). In addition, anti‐GS/FL labels superficial somata of external and internal medullas and internal chiasm cells. Both anti‐GS/20aa‐C and anti‐S100 label heavily the glial sheaths of the lamina ganglionaris. In addition, anti‐S100 binds to the perineurial glia of medullary parenchymal vessels. Western blot analyses show that both anti‐GS/FL and anti‐GS/20aa‐C bind mostly to a band of 50–55 kDa, compatible with a long isoform of vertebrate GS, and accessorily to a possible dimer and, in the case of anti‐GS/20aa‐C, to an ill‐defined band of intermediate mass. Binding of anti‐S100 is selective for a single band

Who reads Regionally specific distribution of the binding of anti-glutamine synthetase and anti-S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn?

Author

Silvana Allodi; Cristine M. Bressan; Sergio L. Carvalho; Leny A. Cavalcante

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2006

Language

EN

Field

Neuroscience (Life Sciences)