Can I read A rapid method based on the one-step reverse transcriptase-polymerase chain reaction (RT-PCR) technique for detection of different strains of citrus tristeza virus on EtoBox?

A rapid method based on the one-step reverse transcriptase-polymerase chain reaction (RT-PCR) technique for detection of different strains of citrus tristeza virus by T. H. Hung; M. L. Wu; H. J. Su is a Agricultural and Biological Sciences article available to read on EtoBox.

What is A rapid method based on the one-step reverse transcriptase-polymerase chain reaction (RT-PCR) technique for detection of different strains of citrus tristeza virus about?

A rapid and sensitive assay based on the one‐step reverse transcriptase‐polymerase chain reaction (RT‐PCR) technique was developed for the detection of citrus tristeza virus (CTV) in citrus plants. This method combines reverse transcription (RT) and polymerase chain reaction (PCR) in one tube using an optimized buffer condition and efficient enzyme mix. In comparison with the two‐step RT‐PCR and enzyme‐linked immunosorbent assay (ELISA) tests, the one‐step RT‐PCR method has a higher sensitivity in CTV detection especially when the CTV concentration is low in its host. After graft‐inoculation, CTV in the infected plant was first monitored by the one‐step RT‐PCR method at the fourth week which is one week earlier than by the two‐step RT‐PCR method and two weeks earlier than by the ELISA test. Three major strains of CTV in Taiwan: seedling yellows (SY), sweet orange stem‐pitting (SO/SP), and pummelo stem‐pitting (Pum/SP) strains, could be accurately detected by this method with a CTV‐specific primer pair. These CTV strains have their different pathogenicities in citrus cultivars grown in Taiwan such as Ponkan mandarin (__Citrus poonensis__ Hort.), Luchen sweet orange (__C. sinensis__

Who reads A rapid method based on the one-step reverse transcriptase-polymerase chain reaction (RT-PCR) technique for detection of different strains of citrus tristeza virus?

Author

T. H. Hung; M. L. Wu; H. J. Su

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0931-1785)

Published

2000

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)