Skip to content

Opening book details…

Can I read Catalytic Mechanism of Human Ten-Eleven Translocation-2 (TET2) Enzyme: Effects of Conformational Changes, Electric Field, and Mutations on EtoBox?

Catalytic Mechanism of Human Ten-Eleven Translocation-2 (TET2) Enzyme: Effects of Conformational Changes, Electric Field, and Mutations by Waheed, Sodiq O. (author);Chaturvedi, Shobhit S. (author);Karabencheva-Christova, Tatyana G. (author);Christov, Christo Z. (author) is a Chemistry article available to read on EtoBox.

What is Catalytic Mechanism of Human Ten-Eleven Translocation-2 (TET2) Enzyme: Effects of Conformational Changes, Electric Field, and Mutations about?

Ten-eleven translocation (TET) family of enzymes are non-heme Fe(II)-and 2-oxoglutarate (2OG)-dependent oxygenases that perform oxidation of the methyl group of the 5-methylcytosine (5mC) on DNA. TET enzymes play a crucial role in epigenetic modifications and have been linked to malignant transformation and various forms of cancer such as prostate, lung, and breast cancer. In this study, molecular dynamic (MD) and combined quantum mechanic/molecular mechanic (QM/MM) approaches were used to explore the catalytic mechanism, conformational dynamics, and the effects of mutations during the first oxidation from 5mC substrate to 5hmC by human TET2 enzyme. The studies reveal that a correlated motion between the main structural elements in TET2, the glycine-serine (GS) linker and the Cys-rich Nterminal (Cys-N) subdomain, plays a key role in the orientation of the DNA substrate in the wild-type (WT) TET2. This correlated motion is affected in the mutant forms of TET2. The conformational changes in the WT TET2 influence the rate of the hydrogen atom abstraction (HAT) step; however, its mechanism via σ-channel remains unchanged. The results enabled us to identify key residues that are crucial

Who reads Catalytic Mechanism of Human Ten-Eleven Translocation-2 (TET2) Enzyme: Effects of Conformational Changes, Electric Field, and Mutations?

It is typically read by researchers, students, and practitioners in Chemistry.

Author
Waheed, Sodiq O. (author);Chaturvedi, Shobhit S. (author);Karabencheva-Christova, Tatyana G. (author);Christov, Christo Z. (author)
Publisher
American Chemical Society (ACS)
Published
2021
Language
EN
Field
Chemistry (Physical Sciences)

More by Waheed, Sodiq O. (author);Chaturvedi, Shobhit S. (author);Karabencheva-Christova, Tatyana G. (author);Christov, Christo Z. (author)

Browse all works by Waheed, Sodiq O. (author);Chaturvedi, Shobhit S. (author);Karabencheva-Christova, Tatyana G. (author);Christov, Christo Z. (author)