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Retention behaviour of beta-blockers in HPLC using a monolithic column by Hisham Hashem; Thomas Jira is a Chemistry article available to read on EtoBox.

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## Retention behaviour of beta-blockers in HPLC using a monolithic column The chromatographic behaviour of a new HPLC-stationary phase (Chromolithm RP-18e) is described for separation of beta-blockers. The effects of the different chromatographic conditions (buffer system, pH value, content of organic modifier, injection volume and flow rate) on the separation behaviour were studied. At higher flow rates the peaks seemed to be more symmetrical than at lower flow rates. The use of buffer or salt in the mobile phase for this separation is found to be very essential and the counter-anion type of this buffer or salt significantly affected the retention behaviour of beta-blockers while the cation type did not play the same important role.

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Author

Hisham Hashem; Thomas Jira

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1615-9306)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)