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DNA interactions of cisplatin tethered to the DNA minor groove binder distamycin by Hana Loskotová; Viktor Brabec is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is DNA interactions of cisplatin tethered to the DNA minor groove binder distamycin about?

Modifications of natural DNA in a cell‐free medium using cisplatin tethered to the AT‐specific, minor groove binder distamycin, were studied using various methods of biochemical analysis or molecular biophysics. These methods include: binding studies using differential pulse polarography and flameless atomic absorption spectrophotometry, mapping DNA adducts using a transcription assay, use of ethidium bromide as a fluorescent probe for DNA adducts of platinum, measurement of DNA unwinding by gel electrophoresis, measurement of CD spectra, an interstrand cross‐linking assay using gel electrophoresis under denaturing conditions, measurement of melting curves with the aid of absorption spectrophotometry and the use of terbium ions as a fluorescent probe for distorted base pairs in DNA. The results indicate that attachment of distamycin to cisplatin changes several features of the DNA‐binding mode of the parent platinum drug. Major differences comprise different conformational alterations in DNA and a considerably higher efficiency of the conjugated drug to form in DNA interstrand cross‐links. Cisplatin tethered to distamycin, however, coordinates to DNA with similar base sequence pref

Who reads DNA interactions of cisplatin tethered to the DNA minor groove binder distamycin?

Author

Hana Loskotová; Viktor Brabec

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)