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This article discusses the challenges of distinguishing cell–cell complexes from singlet cells in non-imaging flow cytometry and single-cell sorting, particularly focusing on T cell-monocyte complexes. The authors highlight that these complexes can lead to misinterpretation of single-cell data, as they exhibit mixed lineage characteristics and can be mistaken for novel cell types. They propose experimental and data analysis strategies, including imaging flow cytometry, to accurately identify these complexes

Author

gargamelyuce

Language

EN