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An Automated High Throughput Filtration Assay: Application to Polymerase Inhibitor Identification by Pengguang Wu; Sarkiz Daniel-Issakani; Kelly LaMarco; Berta Strulovici is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An Automated High Throughput Filtration Assay: Application to Polymerase Inhibitor Identification about?

small molecule drugs that have either broad spectrum A high throughput filtration assay was developed antibacterial or antifungal activity. We used RNA polyand automated for screening compounds and natural merase isolated from Escherichia coli as our primary product extracts using 96-well filterplates. The selecbacterial high throughput target enzyme and contion of a hydrophobic membrane and appropriate capducted a promoter-specific transcription assay. For the ture conditions enabled us to perform the enzymatic fungal polymerase, we used Candida albicans RNA reaction, capture of products, vacuum filtration, and polymerase II as our target. ## detection in a single filtration microplate, without any To develop a high throughput screening assay for transfer step. The hydrophobic membrane has the adpolymerases, we needed to have an automated robotic vantage of preventing any loss of aqueous solution filtration assay. Filtration assays reported in literaduring the enzymatic reaction. The results are compature utilize either a one-plate system with simple vacrable with those obtained in a solid plate format foluum devices (3 -5) or a two-plate system with a cell lowed by transferring t

Who reads An Automated High Throughput Filtration Assay: Application to Polymerase Inhibitor Identification?

Author

Pengguang Wu; Sarkiz Daniel-Issakani; Kelly LaMarco; Berta Strulovici

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)