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The Impact of Anterior Cruciate Ligament Injury on Lubricin Metabolism and the Effect of Inhibiting Tumor Necrosis Factor α on Chondroprotection in an Animal Model by K. A. Elsaid; J. T. Machan; K. Waller; B. C. Fleming; G. D. Jay is a Medicine article available to read on EtoBox.

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## Abstract ## Objective To examine the effects of anterior cruciate ligament transection (ACLT) in a rat model on lubricin metabolism and its relationship to markers of inflammation and cartilage damage, and to determine whether blocking the metabolic effects of tumor necrosis factor α (TNFα) by etanercept increases the chondroprotection provided by lubricin. ## Methods Unilateral ACLT was performed in Lewis rats. Levels of lubricin, TNFα, interleukin‐1β (IL‐1β), and sulfated glycosaminoglycans (sGAG) in synovial fluid (SF) lavage specimens and synovial tissue lubricin gene expression were evaluated at 1 week and 4 weeks following ACLT. Histologic evaluation of articular cartilage included staining with lubricin‐specific monoclonal antibody 9G3 and Safranin O. The percentage of lubricin staining on the surface of articular cartilage in weight‐bearing areas was estimated by digital imaging. Blocking of TNFα was performed using etanercept, which was administered subcutaneously at a dose of 0.5 mg/kg around the ACL‐transected joints, using different dosing strategies. The ACL‐transected and contralateral joints of these rats were harvested 4 weeks following surgery. ## Results Four w

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Author

K. A. Elsaid; J. T. Machan; K. Waller; B. C. Fleming; G. D. Jay

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2009

Language

EN

Field

Medicine (Health Sciences)