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Differential Leukocyte Count Method for Bovine Low Somatic Cell Count Milk by H. Dosogne; F. Vangroenweghe; J. Mehrzad; A.M. Massart-Leën; C. Burvenich is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Differential Leukocyte Count Method for Bovine Low Somatic Cell Count Milk about?

Whereas many differential leukocyte count methods for high somatic cell count (SCC) milk from mastitic cows are available, only a few have been developed for low SCC milk. We have developed a flow cytometric differential leukocyte count method for low SCC milk. The procedure consists of 1) 1.5 ml of diluted milk sample (30%, vol/vol dilution with PBS), 2) centrifugation, 3) leukocyte labeling with SYTO 13 and 4) flow cytometric analysis. Four major leukocyte populations can be clearly identified in the green fluorescence-side scatter dot plot: lymphocytes and monocytes (LM), polymorphonuclear neutrophils (PMN), mature macrophages (MΦ), and cells with apoptotic features based on chromatin condensation and nuclear fragmentation. The optimal processing temperature was 20°C. Significant differences among samples with similar differential leukocyte counts were found. Storage of milk samples during 2 d at 7°C had no effect on differential leukocyte count. Using the new method, differential leukocyte count was performed in low SCC milk samples from cows in early, mid, and late lactation. In accordance with previous studies, PMN and MΦ percentages were lower and LM percentages were higher

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Author

H. Dosogne; F. Vangroenweghe; J. Mehrzad; A.M. Massart-Leën; C. Burvenich

Publisher

American Dairy Science Association

Published

2003

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)