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Rescue of Vitrified-warmed Bovine Mature Oocytes by Short-term Recovery Culture with Resveratrol by Chinen, Shoichiro; Yamanaka, Takahiro; Hirabayashi, Masumi; Hochi, Shinichi is a Medicine article available to read on EtoBox.

What is Rescue of Vitrified-warmed Bovine Mature Oocytes by Short-term Recovery Culture with Resveratrol about?

Resveratrol, a well-known antioxidant, has been reported to protect mouse metaphase-II (M - II) stage oocytes from vitrification injuries when used as a treatment during a series of vitrification processes. The present study was conducted to investigate whether short-term treatment of post-warm bovine mature oocytes with resveratrol can increase blastocyst formation rate following in vitro fertilization and culture. Bovine denuded M - II oocytes were vitrified-warmed using Cryotop® or nylon mesh (pore size = 37 μm) as a cryodevice. The post-warm oocytes were treated for 2 h with 1 μM resveratrol in recovery culture medium. The resveratrol treatment had no harmful influence on morphological survival and cleavage rate of the oocytes vitrified-warmed with Cryotop® or nylon mesh. In the Cryotop® vitrification series, blastocyst formation rate of resveratrol-treated post-warm oocytes (39.0%) was not significantly different from that of non-treated post-warm oocytes (31.7%). However in the nylon mesh vitrification series, there was a significant increase in the blastocyst yield (42.4% vs. 31.3%, P < 0.05) when post-warm oocytes were treated with resveratrol. Blastocyst yield from fresh c

Who reads Rescue of Vitrified-warmed Bovine Mature Oocytes by Short-term Recovery Culture with Resveratrol?

Author

Chinen, Shoichiro; Yamanaka, Takahiro; Hirabayashi, Masumi; Hochi, Shinichi

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0011-2240)

Published

2020

Language

EN

Field

Medicine (Health Sciences)