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Role of α~2~‐Macroglobulin in Regulating Amyloid β‐Protein Neurotoxicity: Protective or Detrimental Factor? by Cinzia Fabrizi; Rita Businaro; Giuliana M. Lauro; Lorenzo Fumagalli is a Neuroscience article available to read on EtoBox.

What is Role of α~2~‐Macroglobulin in Regulating Amyloid β‐Protein Neurotoxicity: Protective or Detrimental Factor? about?

α~2~‐Macroglobulin (α~2~M) has been identified as a carrier protein for β‐amyloid (Aβ) decreasing fibril formation and affecting the neurotoxicity of this peptide. The α~2~‐macroglobulin receptor/low density lipoprotein receptor related protein (LRP) is involved in the internalization and degradation of the α~2~M/Aβ complexes and its impairment has been reported to occur in Alzheimer's disease. Previous studies have shown α~2~M to determine an enhancement or a reduction of Aβ toxicity in different culture systems. In order to clarify the role of α~2~M in Aβ neurotoxicity, we challenged human neuroblastoma cell lines with activated α~2~M in combination with Aβ. Our results show that in neuroblastoma cells expressing high levels of LRP, the administration of activated α~2~M protects the cells from Aβ neurotoxicity. Conversely, when this receptor is not present α~2~M determines an increase in Aβ toxicity as evaluated by MTT and TUNEL assays. In LRP‐negative cells transfected with the full‐length human LRP, the addition of activated α~2~M resulted to be protective against Aβ‐induced neurotoxicity. By means of recombinant proteins we ascribed the neurotoxic activity of α~2~M to its FP3

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Author

Cinzia Fabrizi; Rita Businaro; Giuliana M. Lauro; Lorenzo Fumagalli

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2001

Language

EN

Field

Neuroscience (Life Sciences)