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Mammalian transcription factor LSF is a target of ERK signaling by Zrinka Pagon; Janet Volker; Geoffrey M. Cooper; Ulla Hansen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract LSF is a mammalian transcription factor that is rapidly and quantitatively phosphorylated upon growth induction of resting, peripheral human T cells, as assayed by a reduction in its electrophoretic mobility. The DNA‐binding activity of LSF in primary T cells is greatly increased after this phosphorylation event (Volker et al. [1997]: Genes Dev 11:1435–1446). We demonstrate here that LSF is also rapidly and quantitatively phosphorylated upon growth induction in NIH 3T3 cells, although its DNA‐binding activity is not significantly altered. Three lines of experimentation established that ERK is responsible for phosphorylating LSF upon growth induction in both cell types. First, phosphorylation of LSF by ERK is sufficient to cause the reduced electrophoretic mobility of LSF. Second, the amount of ERK activity correlates with the extent of LSF phosphorylation in both primary human T cells and NIH 3T3 cells. Finally, specific inhibitors of the Ras/Raf/MEK/ERK pathway inhibit LSF modification in vivo. This phosphorylation by ERK is not sufficient for activation of LSF DNA–binding activity, as evidenced both in vitro and in mouse fibroblasts. Nonetheless, activation of ERK is

Who reads Mammalian transcription factor LSF is a target of ERK signaling?

Author

Zrinka Pagon; Janet Volker; Geoffrey M. Cooper; Ulla Hansen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)