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Activity‐dependent somatostatin gene expression is regulated by cAMP‐dependent protein kinase and Ca^2+^‐calmodulin kinase pathways by Isabel Sánchez-Muñoz; Franco Sánchez-Franco; Mario Vallejo; Antonio Fernández; Nuria Palacios; Miriam Fernández; Lucinda Cacicedo is a Neuroscience article available to read on EtoBox.
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## Abstract Ca^2+^ influx through L‐type voltage‐gated Ca^2+^ channels (L‐VSCC) is required for K^+^‐induced somatostatin (SS) mRNA. Increase in intracellular Ca^2+^ concentration leads to the activation of cyclic AMP‐responsive element binding protein (CREB), a key regulator of SS gene transcription. Several different protein kinases possess the capability of driving CREB upon membrane depolarization. We investigated which of the signalling pathways involved in CREB activation mediates SS gene induction in response to membrane depolarization in cerebrocortical cells exposed to 56 mM K^+^. Activity dependent phosphorylation of CREB in Ser^133^ was immunodetected. Activation of CREB was biphasic showing two peaks at 5 and 60 min. The selective inhibitors of extracellular signal related protein kinase/mitogen‐activated protein kinase (ERK/MAPK) PD098059, cyclic‐AMPdependent protein kinase (cAMP/PKA) H89 and RpcAMPS, and Ca^2+^/calmodulin‐dependent protein kinases (CaMKs) pathways KN62 and KN93 were used to determine the signalling pathways involved in CREB activation. Here we show that the early activation of CREB was dependent on cAMP/PKA along with CaMKs pathways whereas the ERK/MA
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- Author
- Isabel Sánchez-Muñoz; Franco Sánchez-Franco; Mario Vallejo; Antonio Fernández; Nuria Palacios; Miriam Fernández; Lucinda Cacicedo
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)
- Published
- 2009
- Language
- EN
- Field
- Neuroscience (Life Sciences)