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Stereoselective analysis of carvedilol in human plasma and urine using HPLC after chiral derivatization by Rosângela G. Peccinini; Valdecir F. Ximenes; Evandro J. Cesarino; Vera L. Lanchote is a Medicine article available to read on EtoBox.
What is Stereoselective analysis of carvedilol in human plasma and urine using HPLC after chiral derivatization about?
## Abstract An enantioselective high‐performance liquid chromatographic method for the analysis of carvedilol in plasma and urine was developed and validated using (‐)‐menthyl chloroformate (MCF) as a derivatizing reagent. Chloroform was used for extraction, and analysis was performed by HPLC on a C18 column with a fluorescence detector. The quantitation limit was 0.25 ng/ml for S(−)‐carvedilol in plasma and 0.5 ng/ml for R(+)‐carvedilol in plasma and for both enantiomers in urine. The method was applied to the study of enantioselectivity in the pharmacokinetics of carvedilol administered in a multiple dose regimen (25 mg/12 h) to a hypertensive elderly female patient. The data obtained demonstrated highest plasma levels for the R(+)‐carvedilol (__AUC__ 75.64 vs 37.29 ng/ml). The enantiomeric ratio R(+)/S(−) was 2.03 for plasma and 1.49 for urine (__Ae__~0–12~ 17.4 vs 11.7 μg). Copyright © 2008 John Wiley & Sons, Ltd.
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- Author
- Rosângela G. Peccinini; Valdecir F. Ximenes; Evandro J. Cesarino; Vera L. Lanchote
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0142-2782)
- Published
- 2008
- Language
- EN
- Field
- Medicine (Health Sciences)