Can I read Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads on EtoBox?

Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads by H. Takeuchi; A. Yasuda; Y. Yasuda-Kamatani; T. Kubo; T. Nakajima is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads about?

## Abstract Using a combination of MALDI‐TOF and on‐line capillary HPLC/Q‐Tof mass spectroscopy, we identified and determined the amino acid sequence of a novel neuropeptide in the brain of the honeybee __Apis mellifera__ L., termed AmTRP peptide (__Apis mellifera__ tachykinin‐related peptide), related to insect tachykinin. A cDNA for a prepro‐protein (prepro‐AmTRP) of AmTRP was isolated and determined to encode seven AmTRPs 1–7. Northern blot analysis indicated that the prepro‐AmTRP gene is expressed differentially in the nurse bee, forager, queen and drone heads. Strong expression was detected in the queen and forager heads, while weak and almost no significant expression was detected in the nurse and drone heads, respectively. These results suggest that AmTRP peptide functions as a neuromodulator and/or hormone, associated with sex‐specific or age/division of labour‐selective behaviour and/or physiology of the honeybees.

Who reads Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads?

Author

H. Takeuchi; A. Yasuda; Y. Yasuda-Kamatani; T. Kubo; T. Nakajima

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0962-1075)

Published

2003

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)