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Role of the Brachial Somites in the Development of the Appendicular Musculature in Rat Embryos by Kenneth K. H. Lee; Lung-Yam Sze is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract DiI, a fluorescent lipophilic dye, was micro‐injected into the brachial somites of 10.5 day rat embryos to determine whether these somites can contribute cells to the development of the fore‐limb bud. The injected embryos were cultured and harvested at the 20–25‐somite stage. The dye did not interfere with somitogenesis because, at the injection site, the DiI‐labelled somites were able to differentiate into dermomyotome and sclerotome. We have analyzed cryo‐sections of 20–21‐somite stage embryos and were unable detect the presence of DiI‐labelled cells in the fore‐limb buds. However, at the 22‐somite stage, a few DiI‐positive cells were found in the proximal region of the limb bud. These labelled cells had migrated into the limb from the lateral border of the dermomyotome. From the 23‐somite stage onwards, there were even more DiI‐positive cells inside the limb. We have performed an additional set of experiments to confirm that the somitic cells do have the ability to invade and colonize the limb bud. This was achieved by first labelling newly formed somites isolated from the caudal region of 10.5 day embryos with DiI and then grafting them into corresponding regions in

Who reads Role of the Brachial Somites in the Development of the Appendicular Musculature in Rat Embryos?

Author

Kenneth K. H. Lee; Lung-Yam Sze

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)