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Alteration of Catecholamine Phenotype in Transgenic Mice Influences Expression of Adrenergic Receptor Subtypes by Kazuto Kobayashi; Akira Ota; Akifumi Togari; Shinji Morita; Tomoko Mizuguchi; Hirohide Sawada; Keiki Yamada; Ikuko Nagatsu; Shosei Matsumoto; Keisuke Fujita; Toshiharu Nagatsu is a Neuroscience article available to read on EtoBox.
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Abstract: Agonist‐induced regulation of adrenergic receptors (ARs) has an important role in controlling physiological functions in response to changes in catecholamine stimulation. We previously generated transgenic mice expressing phenylethanolamine __N__‐methyltransferase (PNMT) under the control of a human dopamine β‐hydroxylase gene promoter to switch catecholamine specificity from the norepinephrine phenotype to the epinephrine phenotype. In the present study, we first examined changes in catecholamine metabolism in peripheral tissues innervated by sympathetic neurons of the transgenic mice. In the transgenic target tissues, a high‐level expression of PNMT led to a dramatic increase in the epinephrine levels, whereas the norepinephrine levels were decreased to 48.6–87.9% of the nontransgenic control levels. Analysis of plasma catecholamines in adrenalectomized mice showed large amounts of epinephrine derived from sympathetic neurons in the transgenic mice. Subsequently, we performed radioligand binding assays with (−)‐[^125^I]iodocyanopindolol to determine changes in binding sites of β‐AR subtypes. In transgenic mice, the number of β2‐AR binding sites was 56.4–74.9% of their n
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- Author
- Kazuto Kobayashi; Akira Ota; Akifumi Togari; Shinji Morita; Tomoko Mizuguchi; Hirohide Sawada; Keiki Yamada; Ikuko Nagatsu; Shosei Matsumoto; Keisuke Fujita; Toshiharu Nagatsu
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1995
- Language
- EN
- Field
- Neuroscience (Life Sciences)