About this document

Exp 5 by Raj Singh is a document available to read on EtoBox.

The document outlines a procedure for separating leaf pigments using Thin Layer Chromatography (TLC), which relies on the differences in solubility and adsorption of pigments. It details the materials required, the steps for preparing the pigment extract and TLC plate, and the expected results, including the identification of distinct pigment bands. The document also includes precautions to ensure accurate results and a method for calculating the Rf value of the pigments.

Author

Raj Singh

Language

EN