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Preparation and Characterization of Hyaluronan Oligosaccharides for Angiogenesis Study by Feng Gao; Manlin Cao; Cuixia Yang; Yiqing He; Yiwen Liu is a Medicine article available to read on EtoBox.

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## Abstract Low molecular weight hyaluronan (HA) is proved to be active in stimulating endothelial cell (EC) proliferation. The study is to obtain biological active HA fragments of defined length from high molecular weight HA for angiogenesis research. A novel method, by a high‐voltage polyacrylamide‐gel electrophoresis (PAGE) that uses specific fluorophore labeling, was developed to identify the degraded products digested from native high molecular weight HA. The polymer HA was digested by hyaluornidase partially, and the enzymatic fragments of HA or HA oligosaccharides (o‐HAs) were analyzed for molecular sizes by a combination of PAGE, high‐performance liquid chromatography (HPLC), and mass spectrometry (MS). The purified HA oligomers were pooled and the biological effects of the mixtures on EC proliferation were studied, by separating the digests of HA fragments by size exclusion chromatography on a Bio‐Gel P‐10 column. The prepared o‐HAs were collected and analyzed by a series of methods, PAGE, HPLC, and MS, which indicated a mixture of oligosaccharides covering a range of different sizes from 4 to 20‐mer. The minimal molecular size determined by the special PAGE was 2 disaccha

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Author

Feng Gao; Manlin Cao; Cuixia Yang; Yiqing He; Yiwen Liu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1552-4973)

Published

2006

Language

EN

Field

Medicine (Health Sciences)