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Can I read EGF-induced redistribution of erbB2 on breast tumor cells: Flow and image cytometric energy transfer measurements on EtoBox?
EGF-induced redistribution of erbB2 on breast tumor cells: Flow and image cytometric energy transfer measurements by Péter Nagy; László Bene; Margit Balázs; William C. Hyun; Steven J. Lockett; Nancy Y. Chiang; Frederick Waldman; Burt G. Feuerstein; Sándor Damjanovich; János Szöllősi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is EGF-induced redistribution of erbB2 on breast tumor cells: Flow and image cytometric energy transfer measurements about?
erbB2, a member of the epidermal growth factor (EGF) receptor-type tyrosine kinase receptor family, is overexpressed in breast carcinomas with poor prognosis. We examined the cell surface association of this receptor with itself and with other cell surface proteins by the Fo ̈rster-type fluorescence resonance energy transfer using whole antibodies and Fab fragments. We found that erbB2 molecules homoassociate in unstimulated SK-BR-3, BT474 and ## BT474-M (a metastatic version of the parent BT474 line) breast tumor cells, and that the interaction was enhanced by EGF treatment in suspensions of SK-BR-3 and BT474-M cells. BT474 cells (with low EGF receptor expression) and attached SK-BR-3 cells do not respond to EGF. Image microscopic energy trans-fer measurements found considerable pixel-by-pixel heterogeneity in the homoassociation state of erbB2. In accordance with the EGF-induced redistribution of erbB2, EGF receptor was found to be in close proximity to erbB2 in FRET measurements. By labeling different epitopes on erbB2 and the lipid bilayer, we were able to prepare an epitope map of erbB2 molecule. Our data suggest the existence of dynamic cell surface patterns of erbB2 and poi
Who reads EGF-induced redistribution of erbB2 on breast tumor cells: Flow and image cytometric energy transfer measurements?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Péter Nagy; László Bene; Margit Balázs; William C. Hyun; Steven J. Lockett; Nancy Y. Chiang; Frederick Waldman; Burt G. Feuerstein; Sándor Damjanovich; János Szöllősi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)