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Identification and preliminary characterization of conserved uncharacterized proteins from Chlamydomonas reinhardtii , Arabidopsis thaliana , and Setaria viridis by Eric P. Knoshaug; Peipei Sun; Ambarish Nag; Huong Nguyen; Erin M. Mattoon; Ningning Zhang; Jian Liu; Chen Chen; Jianlin Cheng; Ru Zhang; Peter St. John; James Umen is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Identification and preliminary characterization of conserved uncharacterized proteins from Chlamydomonas reinhardtii , Arabidopsis thaliana , and Setaria viridis about?
## Abstract The rapid accumulation of sequenced plant genomes in the past decade has outpaced the still difficult problem of genome‐wide protein‐coding gene annotation. A substantial fraction of protein‐coding genes in all plant genomes are poorly annotated or unannotated and remain functionally uncharacterized. We identified unannotated proteins in three model organisms representing distinct branches of the green lineage (Viridiplantae): __Arabidopsis thaliana__ (eudicot), __Setaria viridis__ (monocot), and __Chlamydomonas reinhardtii__ (Chlorophyte alga). Using similarity searching, we identified a subset of unannotated proteins that were conserved between these species and defined them as Deep Green proteins. Bioinformatic, genomic, and structural predictions were performed to begin classifying Deep Green genes and proteins. Compared to whole proteomes for each species, the Deep Green set was enriched for proteins with predicted chloroplast targeting signals predictive of photosynthetic or plastid functions, a result that was consistent with enrichment for daylight phase diurnal expression patterning. Structural predictions using AlphaFold and comparisons to known structures sho
Who reads Identification and preliminary characterization of conserved uncharacterized proteins from Chlamydomonas reinhardtii , Arabidopsis thaliana , and Setaria viridis?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Eric P. Knoshaug; Peipei Sun; Ambarish Nag; Huong Nguyen; Erin M. Mattoon; Ningning Zhang; Jian Liu; Chen Chen; Jianlin Cheng; Ru Zhang; Peter St. John; James Umen
- Publisher
- Wiley
- Published
- 2023
- Field
- Agricultural and Biological Sciences (Life Sciences)