Can I read Borate–nucleotide Complex Formation Depends on Charge and Phosphorylation State on EtoBox?

Borate–nucleotide Complex Formation Depends on Charge and Phosphorylation State by Danny H. Kim; Kym F. Faull; Andrew J. Norris; Curtis D. Eckhert is a Chemistry article available to read on EtoBox.

What is Borate–nucleotide Complex Formation Depends on Charge and Phosphorylation State about?

## Abstract Flow injection analysis with electrospray ionization mass spectrometry was used to investigate borate–nucleotide complex formation. Solutions containing 100 μM nucleotide and 500 μM boric acid in water–acetonitrile–triethylamine (50 : 50 : 0.2, v/v/v; pH 10.3) showed that borate complexation with nicotinamide nucleotides was significantly influenced by the charge on the nicotinamide group and the number of phosphate groups on the adenine ribose. Borate binding decreased in the order of NAD^+^, NADH, NADP^+^ and NADPH. To investigate the relationship between complex formation and phosphorylation, association constants (__K__~A~) of borate–adenine (AMP, ADP, ATP), –guanine (GMP, GDP, GTP), –cytidine (CMP, CDP, CTP) and –uridine (UMP, UDP, UTP) complexes were compared. The results showed that the number of nucleotide phosphate groups was inversely proportional to the relative abundance of the borate complexes, with the __K__~A~ of borate–nucleotide complex decreasing in the order mono‐, di‐ and tri‐phosphates (AMP ≈ GMP ≈ CMP ≈ UMP > ADP ≈ GDP ≈ CDP ≈ UDP > GTP > ATP ≈ CTP ≈ UTP). At pH 7.4, using ammonium bicarbonate buffer, only borate–NAD^+^ complex was observed. This i

Who reads Borate–nucleotide Complex Formation Depends on Charge and Phosphorylation State?

Author

Danny H. Kim; Kym F. Faull; Andrew J. Norris; Curtis D. Eckhert

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

2004

Language

EN

Field

Chemistry (Physical Sciences)