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Cosubstrate effect on xylose reductase and xylitol dehydrogenase activity levels, and its consequence on xylitol production by Candida tropicalis by Elena Tamburini; Ercolina Bianchini; Alessandro Bruni; Giuseppe Forlani is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Due to its anticariogenic properties, the five-carbon sugar alcohol xylitol is finding increasing use in food industry as a functional sweetener. Although xylitol is currently manufactured by the chemical reduction of xylose, its biological production would be attractive because of lower costs and better organoleptic features. In xylose-fermenting yeasts, the substrate is directly converted to xylitol by an NAD(P)H-dependent reductase (XR). Xylitol is oxidized in turn by an NAD + -dependent dehydrogenase (XDH) yielding xylulose, which is channelled into the pentose phosphate pathway. In the absence of other carbon sources, a significant fraction of xylitol is used to fuel cell growth. To avoid excess yield loss and maximize xylitol production, a series of mono-and disaccharides were tested as cosubstrates for a hyper-acidophilic strain of Candida tropicalis. Their effect upon XR and XDH specific activity levels was evaluated, and the resulting expression of the inducible pathway and the XR:XDH ratio were related to either non-optimized or optimized yields. Glucose strongly inhibited xylose reduction, whereas galactose stimulated xylitol utilization. On the contrary, the addition of

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Author

Elena Tamburini; Ercolina Bianchini; Alessandro Bruni; Giuseppe Forlani

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)