Can I read Cryotechniques in Biological Electron Microscopy || on EtoBox?

Cryotechniques in Biological Electron Microscopy || by Luis Bachmann, Erwin Mayer (auth.), Prof. Dr. Rudolf Alexander Steinbrecht, Dr. Karl Zierold (eds.) is a nonfiction available to read on EtoBox.

What is Cryotechniques in Biological Electron Microscopy || about?

To preserve tissue by freezing is an ancient concept going back pre­ sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan­ ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro­ molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos­ sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro­ duced a wealth of successful applications.

Who reads Cryotechniques in Biological Electron Microscopy ||?

Author

Luis Bachmann, Erwin Mayer (auth.), Prof. Dr. Rudolf Alexander Steinbrecht, Dr. Karl Zierold (eds.)

Publisher

Springer Berlin / Heidelberg

Published

1987

Language

EN

ISBN

9783642728150

Category

nonfiction

Subjects

Science, Technology, Medical