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Can I read Interaction of DAPI with individual strands of trinucleotide repeats : Effects on replication in vitro of the AAT·ATT triplet on EtoBox?
Interaction of DAPI with individual strands of trinucleotide repeats : Effects on replication in vitro of the AAT·ATT triplet by Edoardo Trotta; Nicoletta Del Grosso; Maura Erba; Sonia Melino; Daniel Cicero; Maurizio Paci is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Interaction of DAPI with individual strands of trinucleotide repeats : Effects on replication in vitro of the AAT·ATT triplet about?
The structural changes produced by the minor‐groove binding ligand DAPI (4′,6‐diamidine‐2‐phenylindole) on individual strands of trinucleotide repeat sequences were detected by electrophoretic band‐shift analysis and related to their effects on DNA replication __in vitro__. Among the 20 possible single‐stranded trinucleotide repeats, only the T‐rich strand of the AAT·ATT triplet exhibits an observable fluorescence band and a change in electrophoretic mobility due to the drug binding. This is attributable to the property of DAPI that favours folding of the random coil ATT strand into a fast‐migrating hairpin structure by a minor‐groove binding mechanism. Electrophoretic characteristics of AAT, ACT, AGT, ATG and ATC are unchanged by DAPI, suggesting the crucial role of T·T with respect to A·A, C·C and G·G mismatch, in favouring the binding properties and the structural features of the ATT–DAPI complexes. Primer extension experiments, using the Klenow fragment of DNA polymerase I, demonstrate that such a selective structural change at ATT targets presents a marked property to stall DNA replication __in vitro__ in comparison with the complementary AAT and a random GC‐rich sequence. The
Who reads Interaction of DAPI with individual strands of trinucleotide repeats : Effects on replication in vitro of the AAT·ATT triplet?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Edoardo Trotta; Nicoletta Del Grosso; Maura Erba; Sonia Melino; Daniel Cicero; Maurizio Paci
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)