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Proteomic identification of a role for the von Hippel Lindau tumour suppressor in changes in the expression of mitochondrial proteins and septin 2 in renal cell carcinoma by Rachel A. Craven; Sarah Hanrahan; Nick Totty; Patricia Harnden; Anthea J. Stanley; Eamonn R. Maher; Adrian L. Harris; William S. Trimble; Peter J. Selby; Rosamonde E. Banks is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Proteomic identification of a role for the von Hippel Lindau tumour suppressor in changes in the expression of mitochondrial proteins and septin 2 in renal cell carcinoma about?
## Abstract The von Hippel Lindau (VHL) tumour suppressor gene, __VHL__, plays a central role in development of sporadic conventional renal cell carcinomas (RCCs). Studying VHL function may, therefore, increase understanding of the pathogenesis of RCC and identify markers/therapeutic targets. Comparison of 2‐DE protein profiles of __VHL__‐defective RCC cells (UMRC2) transfected with control vector or wild‐type __VHL__ showed differences in 30 proteins, including several novel changes. One of the findings confirmed by Western blotting was up‐regulation of the mitochondrial protein ubiquinol cytochrome c reductase complex core protein 2 following VHL transfection, a change that was also observed in two other cell line backgrounds. A marked decrease in expression of this and several other mitochondrial proteins was demonstrated in RCC tissues and using VHL‐transfectants, several were shown to exhibit VHL‐dependent regulation. Thus, VHL may contribute to the decreased mitochondrial function seen in RCC. A form of septin 2 down‐regulated following VHL transfection was also identified. Septin 2 was up‐regulated in 12/16 RCCs, while alteration of the form present was also observed in 1/3
Who reads Proteomic identification of a role for the von Hippel Lindau tumour suppressor in changes in the expression of mitochondrial proteins and septin 2 in renal cell carcinoma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rachel A. Craven; Sarah Hanrahan; Nick Totty; Patricia Harnden; Anthea J. Stanley; Eamonn R. Maher; Adrian L. Harris; William S. Trimble; Peter J. Selby; Rosamonde E. Banks
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)