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An Ion Chromatography–Ultrahigh-Resolution-MS^1^/Data-Independent High-Resolution MS^2^ Method for Stable Isotope-Resolved Metabolomics Reconstruction of Central Metabolic Networks by Qiushi Sun; Teresa W.-M. Fan; Andrew N. Lane; Richard M. Higashi is a scholarly article available to read on EtoBox.

What is An Ion Chromatography–Ultrahigh-Resolution-MS^1^/Data-Independent High-Resolution MS^2^ Method for Stable Isotope-Resolved Metabolomics Reconstruction of Central Metabolic Networks about?

The metabolome comprises a complex network of interconnecting enzyme-catalyzed reactions that involve transfers of numerous molecular subunits. Thus, the reconstruction of metabolic networks requires metabolite substructures to be tracked. Subunit tracking can be achieved by tracing stable isotopes through metabolic transformations using NMR and ultrahigh -resolution (UHR)-mass spectrometry (MS). UHR-MS 1 readily resolves and counts isotopic labels in metabolites but requires tandem MS to help identify isotopic enrichment in substructures. However, it is challenging to perform chromatography-based UHR-MS 1 with its long acquisition time, while acquiring MS 2 data on many coeluting labeled isotopologues for each metabolite. We have developed an ion chromatography (IC)-UHR-MS 1 /data-independent(DI)-HR-MS 2 method to trace the fate of 13 C atoms from [ 13 C 6 ]-glucose ([ 13 C 6 ]-Glc) in 3D A549 spheroids in response to anticancer selenite and simultaneously 13 C/ 15 N atoms from [ 13 C 5 , 15 N 2 ]-glutamine ([ 13 C 5 , 15 N 2 ]-Gln) in 2D BEAS-2B cells in response to arsenite transformation. This method retains the complete isotopologue distributions of metabolites via UHR-MS 1 wh

Author

Qiushi Sun; Teresa W.-M. Fan; Andrew N. Lane; Richard M. Higashi

Publisher

American Chemical Society (ACS)

Published

2021

Language

EN