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Experimental infection by Toxoplasma gondii using contaminated semen containing different doses of tachyzoites in sheep by Érica Paes Barreto Xavier de Moraes; André Mariano Batista; Eduardo Bento Faria; Roberta Lemos Freire; Antonio Carlos Freitas; Maria Angélica Ramos Silva; Valdir A. Braga; Rinaldo Aparecido Mota is a Immunology and Microbiology article available to read on EtoBox.
What is Experimental infection by Toxoplasma gondii using contaminated semen containing different doses of tachyzoites in sheep about?
Toxoplasmosis is a parasitic disease caused by Toxoplasma gondii that affects reproductive performance in small ruminants. Although the T. gondii life cycle is well understood since 1960s, several aspects related to its infection remain unclear. In the present study we hypothesized that sheep inseminated with T. gondii-contaminated semen would develop toxoplasmosis. In order to test that hypothesis, 41 sheep were experimentally infected with semen spiked with the organism. Females were divided in three groups (G1-G3): (a) females in G1 group were inseminated with semen containing 6.5 x 10(4) tachyzoites; (b) females in G2 group with semen containing 4 x 10(7) tachyzoites; and (c) females in G3 group with tachyzoite-free semen (control group). To confirm T. gondii infection via semen, serological tests were performed using indirect immunofluorescence reaction and the detection of parasite DNA in the blood stream using the nested PCR test. While in G1 group only 5/15 (33.3%) of the females presented seroconversion, all sheep in G2 15/15 (100%) seroconverted. The nested PCR test showed that 14/15 (93.3%) of the females in the G1 and 14/15 (93.3%) in the G2 group were positive for T. g
Who reads Experimental infection by Toxoplasma gondii using contaminated semen containing different doses of tachyzoites in sheep?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Érica Paes Barreto Xavier de Moraes; André Mariano Batista; Eduardo Bento Faria; Roberta Lemos Freire; Antonio Carlos Freitas; Maria Angélica Ramos Silva; Valdir A. Braga; Rinaldo Aparecido Mota
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)
- Published
- 2010
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)