Can I read Lactate modulates microglial inflammatory responses after oxygen-glucose deprivation through HIF-1α-mediated inhibition of NF-κB on EtoBox?

Lactate modulates microglial inflammatory responses after oxygen-glucose deprivation through HIF-1α-mediated inhibition of NF-κB by Yuanyuan Zhang; Pengyu Jia; Kui Wang; Yan Zhang; Yuying Lv; Pei Fan; Liufei Yang; Shuyue Zhang; Tianyue Wang; Jing Zhao; Haixia Lv; Xinlin Chen; Yong Liu; Haidong Wei; Pengbo Zhang is a scholarly article available to read on EtoBox.

What is Lactate modulates microglial inflammatory responses after oxygen-glucose deprivation through HIF-1α-mediated inhibition of NF-κB about?

Metabolic adaption drives microglial inflammatory responses, and lactate shapes immunological and inflammatory states. However, whether lactate was involved in the regulation of microglial inflammatory responses after cerebral ischemia remains unclear. In this study, the expression of iNOS, arginase-1, phosphorylated NF-κB p65 and IκB-α, and HIF-1α in BV2 cells after oxygen-glucose deprivation (OGD) were detected by western blotting and immunofluorescence. The mRNA levels of microglial responsive markers and inflammatory factors were assessed by real-time-qPCR. The effect of lactate-treated BV2 cells on the survival of primary neurons was observed using transwell co-culture. The results showed that the protein levels of iNOS and arginase-1, the ratio of mRNA levels of iNOS/CD206, CD86/Ym1, IL-6/IL-10, TNF-α/IL-10 and the mRNA levels of IL-6 and TNF-α, as well as the protein levels of phosphorylated NF-κB p65 and IκB-α, were increased after OGD. Lactate treatment inhibited the OGD-induced increase in the protein levels of iNOS, phosphorylated NF-κB p65 and IκB-α, as well as iNOS/CD206, CD86/Ym1, IL-6/IL-10, TNF-α/IL-10, IL-6 and TNF-α mRNA levels in BV2 cells, while promoted arginas

Author

Yuanyuan Zhang; Pengyu Jia; Kui Wang; Yan Zhang; Yuying Lv; Pei Fan; Liufei Yang; Shuyue Zhang; Tianyue Wang; Jing Zhao; Haixia Lv; Xinlin Chen; Yong Liu; Haidong Wei; Pengbo Zhang

Publisher

Elsevier BV

Published

2023

Language

EN