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Novel Determination of the Influence of Idarubicin upon DNA Chain Structure Using an Electrochemical DNA Biosensor by Voltammetry by Hasret Subak is a Chemistry article available to read on EtoBox.

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Two sequences of DNA (single-strand DNA (ssDNA) and doublestrand DNA (dsDNA)) modified disposable electrodes were designed to investigate the effect of antitumor agent drug idarubicine (IDA) on the DNA chain structure. The effect of IDA on DNA structure was analyzed by square wave voltammetry (SWV) depending on not only the guanine signal but also IDA oxidation response. The present study included the electrochemical investigation of IDA and the investigation of the biomolecular interaction between IDA and DNA. IDA was detected in buffer and urine using disposable pencil graphite electrodes (PGE) with SWV and cyclic voltammetry (CV)). The detection limit (LOD) of IDA in urine samples was 0.089 mg mL -1 with SWV under optimum conditions. For the biomolecular interaction of IDA and DNA, all electrochemical conditions, such as the concentration of DNA, concentration of IDA, interaction phase (at electrode surface/in solution phase), the interaction pH, and the interaction time were optimized. IDA interacted biomolecularly with DNA at the electrode surface and in solution phase using two methods. There is no previous electrochemical study performed on the interaction of IDA with ssDNA.

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Author

Hasret Subak

Publisher

Informa UK Limited

Published

2024

Language

EN

Field

Chemistry (Physical Sciences)