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A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections by Guanbo Wang; Lena Glaser; Nichollas E. Scott; Yasmine Fathy Mohamed; Rebecca Ingram; Karine Laroucau; Miguel A. Valvano is a Medicine article available to read on EtoBox.
What is A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections about?
We recently described a protein O-glycosylation pathway conserved in all species of the Burkholderia genus that results in the synthesis and incorporation of a trisaccharide glycan to membrane-exported proteins. Here, we exploited this system to construct and evaluate a diagnostic tool for glanders. Burkholderia mallei, the causative agent of glanders, is a highly infectious and fatal zoonotic pathogen that infects horses, mules, donkeys, and occasionally humans. A highly sensitive and specific diagnostic tool is crucial for the control, elimination, and eradication of B. mallei infections. We constructed plasmids carrying synthetic genes encoding a modified, previously unannotated Burkholderia glycoprotein containing three glycosylation sequons fused to the cholera toxin B-subunit. The resulting proteins were glycosylated in the B. cenocepacia K56-2 parental strain, but not in glycosylationdeficient mutants, as determined by SDS-PAGE and fluorescent lectin blots. One of these glycoproteins was used as an antigen in ELISA and western blots to screen a panel of serum samples collected from glanders-infected and healthy horses, which were previously investigated by complement fixatio
Who reads A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections?
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- Author
- Guanbo Wang; Lena Glaser; Nichollas E. Scott; Yasmine Fathy Mohamed; Rebecca Ingram; Karine Laroucau; Miguel A. Valvano
- Publisher
- Informa UK Limited
- Published
- 2021
- Language
- EN
- Field
- Medicine (Life Sciences)