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Computational Modeling of Cooperative Regulation of Both Strands in Thin Filaments by Yun Shi; Sarah A. Kosta; Jose R. Pinto; Vitold E. Galkin; Kenneth S. Campbell; Prescott B. Chase is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Computational Modeling of Cooperative Regulation of Both Strands in Thin Filaments about?

The heart's pumping capacity is determined by myofilament power generation. Power is work done per unit time and measured as the product of force and velocity. At a sarcomere level, these contractile properties are linked to the number of attached cross-bridges and their cycling rate; and many signaling pathways modulate one or both of these factors. We previously showed that rodent permeabilized cardiac myocyte power is increased following PKA-mediated phosphorylation of myofibrillar proteins (Herron, Korte, McDonald, Circ. Res. 2001). This increase in power demonstrates a capacity for improved myofilament function and this therapeutic potential adds significance for identification of the molecular specificity underlying enhanced power output. To address molecular specificity at the thin filament level, myofilament mechanical properties were measured before and after exchange of endogenous troponin with recombinant human Tn complex, which contained cardiac (c)TnT, cTnC and either WT cTnI or pseudophosphorylated cTnI at sites Ser23/24Asp, Y26E, or the combinatorial Ser23/24Asp and Y26E in single permeabilized rat slow-twitch skeletal muscle fibers. We found that cTnI Ser23/24Asp, Y

Who reads Computational Modeling of Cooperative Regulation of Both Strands in Thin Filaments?

Author

Yun Shi; Sarah A. Kosta; Jose R. Pinto; Vitold E. Galkin; Kenneth S. Campbell; Prescott B. Chase

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)